The field of genome editing is experiencing a renaissance driven primarily by the repurposing of an immune response system utilized by bacteria and archaea. This system is characterized by the presence of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) stored in host genomes as memories of phage-bacteria interactions. The CRISPR constituents include Cas9, an endonuclease protein, and a guide RNA (sgRNA, or crRNA and tracrRNA in native systems). After forming a Cas9/sgRNA complex and upon recognition of the Protospacer Adjacent Motif (PAM) in a target DNA, endonuclease activity is triggered. The use of the CRISPR-Cas9 system in higher organisms (including mammalian cells and animal models) has spurred myriads of applications critically relevant to agriculture, biomanufacturing, and human health.

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Selected news
1. Market size: URL
2. What is genome editing: URL

Recent patents from our lab
1. Self-cleaved AAV vectors: URL
2. TALE libraries: URL

Reviews from our lab
1. Engineered transcription factors: URL
2. Guide RNA engineering: URL
3. Transcription Activator-like Effectors: URL