Receptor binding

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The binding of a radioligand to a recepter preparation normally involves a ³nonspecific² component in addition to the ³specific² receptor binding. In principle, at least, ³specific² binding can be estimated from the total binding (T) by subracting ³nonspecific² binding (NB). (Theoretical curves with Bmax = 5.6 fmol/mg protein, KL = 45 nM, and c = 0.0083 fmol/nM.)